Year 2025 / Volume 117 / Number 6
Original
Increasing miR-1260b predicts the risk of gastric cancer in atrophic gastritis patients and regulates cell growth and metastasis of gastric cancer

327-335

DOI: 10.17235/reed.2024.10527/2024

Yesheng Huang, Xiayang Lu, Yu Wang, Yichuan Zhang, Meini Cen,

Abstract
Background: atrophic gastritis has a high risk of progressing to gastric cancer. Screening early gastric cancer and predicting the risk of atrophic gastritis developing into gastric cancer could improve the prognosis. Objective: this study evaluated the significance of miR-1260b in early gastric cancer and the progression of atrophic gastritis to gastric cancer aiming to explore a reliable biomarker. Materials and methods: the study enrolled 78 early gastric cancer patients and 77 atrophic gastritis patients. The expression of miR-1260b was detected in serum and tissue samples by PCR. The risk of atrophic gastritis patients progressing to gastric cancer was assessed and correlated with miR-1260b levels. The potential of miR-1260b to distinguish early gastric cancer was evaluated by ROC. In vitro, gastric cancer cells were infected with Helicobacter pylori (H. pylori), and the regulatory effect of miR-1260b on cell growth and metastasis was evaluated by CCK8 and Transwell assay. Results: significant upregulation of miR-1260b was observed in early gastric cancer patients relative to atrophic gastritis patients, which distinguishes early gastric cancer patients and showed a positive correlation with the risk of atrophic gastritis patients developing gastric cancer. Early gastric cancer patients with positive H. pylori infection had higher miR-1260b levels, and increasing miR-1260b was also observed in H. pylori-infected gastric cancer cells. H. pylori promoted cell growth and metastasis of gastric cancer while silencing miR-1260b alleviated these effects. miR-1260b negatively regulated ZNF302, and the knockdown of ZNF302 reversed the protective effect of miR-1260 knockdown on gastric cancer cells. Conclusion: increasing miR-1260b can assist in the early diagnosis of gastric cancer and predict the risk of gastric cancer in atrophic gastritis patients. Silencing miR-1260b may alleviate the promotion of gastric cancer induced by H. pylori via negative modulation of ZNF302.
Lay Summary
1. miR-1260b was previously screened of great potential to diagnose gastric cancer, and its was demonstrated to be involved in the development of colorectal cancer and non-small cell lung cancer. 2. This study revealed the predictive value of miR-1260b in early gastric cancer and tumor progression. 3. Silencing miR-1260b could alleviate the promotion of gastric cancer induced by H. pylori via negatively modulating ZNF302. 4. Increasing miR-1260b can be considered a diagnostic biomarker for early gastric cancer predicting cancer risk and mediating tumor progression.
New comment
Comments
No comments for this article
References
[1] Han Z, Liu J, Zhang W, et al. Cardia and non-cardia gastric cancer risk associated with Helicobacter pylori in East Asia and the West: A systematic review, meta-analysis, and estimation of population attributable fraction. Helicobacter. 2023;28(2):e12950.
[2] López MJ, Carbajal J, Alfaro AL, et al. Characteristics of gastric cancer around the world. Crit Rev Oncol Hematol. 2023;181103841.
[3] Xie T, Zhang Z, Peng Z Shen L. Next stage of collaboration in East Asia gastric cancer treatment. Chin Clin Oncol. 2023;12(4):39.
[4] Yao K, Uedo N, Kamada T, et al. Guidelines for endoscopic diagnosis of early gastric cancer. Dig Endosc. 2020;32(5):663-98.
[5] Young E, Philpott H Singh R. Endoscopic diagnosis and treatment of gastric dysplasia and early cancer: Current evidence and what the future may hold. World J Gastroenterol. 2021;27(31):5126-51.
[6] Lee YC, Chiang TH, Chou CK, et al. Association Between Helicobacter pylori Eradication and Gastric Cancer Incidence: A Systematic Review and Meta-analysis. Gastroenterology. 2016;150(5):1113-24.e5.
[7] Watari J, Chen N, Amenta PS, et al. Helicobacter pylori associated chronic gastritis, clinical syndromes, precancerous lesions, and pathogenesis of gastric cancer development. World J Gastroenterol. 2014;20(18):5461-73.
[8] Shin VY Chu KM. MiRNA as potential biomarkers and therapeutic targets for gastric cancer. World J Gastroenterol. 2014;20(30):10432-9.
[9] Wang QX, Zhu YQ, Zhang H Xiao J. Altered MiRNA expression in gastric cancer: a systematic review and meta-analysis. Cell Physiol Biochem. 2015;35(3):933-44.
[10] Yao Y, Ding Y, Bai Y, et al. Identification of Serum Circulating MicroRNAs as Novel Diagnostic Biomarkers of Gastric Cancer. Front Genet. 2020;11591515. https://www.ncbi.nlm.nih.gov/pubmed/33597967.
[11] Kim DH, Park H, Choi YJ, et al. Exosomal miR-1260b derived from non-small cell lung cancer promotes tumor metastasis through the inhibition of HIPK2. Cell Death Dis. 2021;12(8):747.
[12] Ren J, Wang D, Huang H, et al. miR-1260b Activates Wnt Signaling by Targeting Secreted Frizzled-Related Protein 1 to Regulate Taxane Resistance in Lung Adenocarcinoma. Front Oncol. 2020;10557327.
[13] Xu L, Li L, Li J, et al. Overexpression of miR-1260b in Non-small Cell Lung Cancer is Associated with Lymph Node Metastasis. Aging Dis. 2015;6(6):478-85.
[14] Xu L, Xu X, Huang H, et al. MiR-1260b promotes the migration and invasion in non-small cell lung cancer via targeting PTPRK. Pathol Res Pract. 2018;214(5):776-83.
[15] Liu DR, Guan QL, Gao MT, Jiang L Kang HX. miR-1260b is a Potential Prognostic Biomarker in Colorectal Cancer. Med Sci Monit. 2016;222417-23. https://www.ncbi.nlm.nih.gov/pubmed/27399918.
[16] Park S, Kim J, Cho Y, et al. Promotion of tumorigenesis by miR-1260b-targeting CASP8: Potential diagnostic and prognostic marker for breast cancer. Cancer Sci. 2022;113(6):2097-108. https://www.ncbi.nlm.nih.gov/pubmed/35325509.
[17] Cheung DY. Atrophic Gastritis Increases the Risk of Gastric Cancer in Asymptomatic Population in Korea. Gut Liver. 2017;11(5):575-76.
[18] Lahner E, Conti L, Annibale B Corleto VD. Current Perspectives in Atrophic Gastritis. Curr Gastroenterol Rep. 2020;22(8):38.
[19] Garcés-Durán R, Llach J, Da Fieno A, Córdova H Fernández-Esparrach G. Endoscopic diagnosis of H. pylori infection. Gastroenterol Hepatol. 2023;46(6):483-88.
[20] Lunn JA Weinstein WM. Gastric biopsy. Gastrointest Endosc Clin N Am. 2000;10(4):723-38, vii.
[21] Sayed D Abdellatif M. MicroRNAs in development and disease. Physiol Rev. 2011;91(3):827-87. https://www.ncbi.nlm.nih.gov/pubmed/21742789.
[22] Sun DZ, Liu L, Jiao JP, et al. Syndrome characteristics of traditional Chinese medicine: summary of a clinical survey in 767 patients with gastric cancer. Zhong Xi Yi Jie He Xue Bao. 2010;8(4):332-40.
[23] Yoshimura Y, Yasutake K, Imamura Y, Sashikata T Oimomi M. Endoscopic studies on the superficial spreading type of early gastric cancer. Kobe J Med Sci. 1989;35(1):29-38.
[24] Kuipers EJ. Helicobacter pylori and the risk and management of associated diseases: gastritis, ulcer disease, atrophic gastritis and gastric cancer. Aliment Pharmacol Ther. 1997;11 Suppl 171-88.
[25] Lim NR Chung WC. Helicobacter pylori-associated Chronic Atrophic Gastritis and Progression of Gastric Carcinogenesis. Korean J Gastroenterol. 2023;82(4):171-79.
[26] Demiryas S, Kocazeybek B, Demirci M, et al. Helicobacter pylori-miRNA interaction in gastric cancer tissues: First prospective study from Turkey. New Microbiol. 2019;42(4):210-20.
[27] Hirata H, Hinoda Y, Shahryari V, et al. Genistein downregulates onco-miR-1260b and upregulates sFRP1 and Smad4 via demethylation and histone modification in prostate cancer cells. Br J Cancer. 2014;110(6):1645-54. https://www.ncbi.nlm.nih.gov/pubmed/24504368.
[28] Jiang T, Huang M, Jiang T, et al. Genome-wide compound heterozygosity analysis highlighted 4 novel susceptibility loci for congenital heart disease in Chinese population. Clin Genet. 2018;94(3-4):296-302.
[29] Lin X, Wang S, Lin K, et al. Competitive Endogenous RNA Landscape in Epstein-Barr Virus Associated Nasopharyngeal Carcinoma. Front Cell Dev Biol. 2021;9782473.
[30] Reus LM, Pasaniuc B, Posthuma D, et al. Gene Expression Imputation Across Multiple Tissue Types Provides Insight Into the Genetic Architecture of Frontotemporal Dementia and Its Clinical Subtypes. Biol Psychiatry. 2021;89(8):825-35.
Related articles

Special Article

V Spanish Consensus Conference on Helicobacter pylori infection treatment

DOI: 10.17235/reed.2021.8358/2021

Letter to the Editor

A question about dyspeptic patients analyzed using psychometric scores

DOI: 10.17235/reed.2017.5401/2017

Letter to the Editor

Acute psychotic episode secondary to Helicobacter pylori eradication treatment

DOI: 10.17235/reed.2017.4482/2016

Citation tools
Huang Y, Lu X, Wang Y, Zhang Y, Cen M. Increasing miR-1260b predicts the risk of gastric cancer in atrophic gastritis patients and regulates cell growth and metastasis of gastric cancer. 10527/2024


Download to a citation manager

Download the citation for this article by clicking on one of the following citation managers:

Metrics
This article has received 77 visits.
This article has been downloaded 6 times.

Statistics from Dimensions


Statistics from Plum Analytics

Publication history

Received: 10/05/2024

Accepted: 02/12/2024

Online First: 09/01/2025

Published: 10/06/2025

Article revision time: 197 days

Article Online First time: 244 days

Article editing time: 396 days


Share
This article hasn't been rated yet.
Reader rating:
Valora este artículo:




Asociación Española de Ecografía Digestiva Sociedad Española de Endoscopia Digestiva Sociedad Española de Patología Digestiva
The Spanish Journal of Gastroenterology is the official organ of the Sociedad Española de Patología Digestiva, the Sociedad Española de Endoscopia Digestiva and the Asociación Española de Ecografía Digestiva
Cookie policy Privacy Policy Legal Notice © Copyright 2025 y Creative Commons. The Spanish Journal of Gastroenterology