Year 2019 / Volume 111 / Number 10
Original
Clinical characteristics and genetic profiles of young and adult patients with cholestatic liver disease

775-788

DOI: 10.17235/reed.2019.6168/2019

Minh Tuan Huynh, Truong-Tam Nguyen, Sophie Grison, Olivier Lascols, Eric Fernandez, Véronique Barbu,

Abstract
Background and aims: heterozygous ABCB4, ABCB11 and ATP8B1 sequence variants were previously reported to be associated with low phospholipid-associated cholelithiasis, intrahepatic cholestasis of pregnancy, benign recurrent intrahepatic cholestasis and biliary lithiasis. The present study aimed to identify the presence of sequence variations in genes responsible for Mendelian liver disorders in patients with cholestatic liver disease. Methods: targeted massive parallel sequencing of a panel of genes involved in bile acid homeostasis was performed in 105 young and adult patients with cholestatic liver disease in our laboratory for molecular diagnosis. The effects of novel variants were evaluated using bioinformatics prediction tools and the Protter and Phyre2 software programs were used to create 2D, 3D topology protein modeling. Genotype-phenotype correlation was established according to molecular analysis and clinical records. Results: twenty novel heterozygous ABCB4 sequence variations, one heterozygous ABCB4 large intragenic deletion and only one novel missense variant in ABCB11 and ATP8B1 were identified. Interestingly, heterozygous and homozygous SLC4A2 missense variants were detected in patients with low phospholipid-associated cholelithiasis. Two patients harbored heterozygous GPBAR1 variants. Common variants such as homozygous ABCB11 p.Val444Ala and heterozygous ABCG8 p.Asp19His were also identified in 12 cases. Conclusions: forty-eight variants were identified in five genes including ABCB4, ABCB11, ATP8B1, SLC4A2 and GPBAR1, twenty-five of which were novel. This study expands the phenotypic and mutational spectrum in genes involved in bile acid homeostasis and highlights the genetic and phenotypic heterogeneity in patients with inherited liver disorders.
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Huynh M, Nguyen T, Grison S, Lascols O, Fernandez E, Barbu V, et all. Clinical characteristics and genetic profiles of young and adult patients with cholestatic liver disease. 6168/2019


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Publication history

Received: 10/01/2019

Accepted: 17/04/2019

Online First: 20/09/2019

Published: 03/10/2019

Article revision time: 83 days

Article Online First time: 253 days

Article editing time: 266 days


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