Year 2019 / Volume 111 / Number 4
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Year 2019 / Volume 111 / Number 4


Metabolic characterization of two different non-alcoholic fatty liver disease pre-clinical mouse models


Rocío Gallego-Durán, Leticia Álvarez-Amor, Antonio Gil-Gómez, Ángela Rojas, Rocío Muñoz-Hernández, Antonio Cádernas-García, Douglas Maya-Miles, Rocío Montero-Vallejo, Sheila Gato, Yolanda Sánchez Torrijos, Javier Ampuero, Francisco Martín, Manuel Romero-Gómez,

Introduction: non-alcoholic fatty liver disease is one of the most prevalent liver disorders in the developed world. Currently, there is no approved pharmacological therapy except for lifestyle intervention. Therefore, there is a need to increase the knowledge of preclinical models in order to boost novel discoveries that could lead to a better therapeutic management. Material and methods: this study characterized the effects of two different diets, a long-term high-fat high-fructose diet (HF-HFD) and a choline-deficient, methionine supplemented high-fat diet (CDA-HFD) in C57BL/6J mice for 52 weeks or 16 weeks, respectively. Body weight, lipid and hepatic profile were analyzed and liver histology was subsequently evaluated. Results: HF-HFD animals had an increased body weight and total cholesterol levels, whereas the opposite occurred in CDA-HFD. Both HF-HFD and CDA-HFD animals had higher ALT and AST levels. With regard to histology findings, HF-HFD and CDA-HFD diets induced an increased collagen deposit and intrahepatic steatosis accumulation. Conclusion: in conclusion, the comparison of these models aided in the selection of a long-term, more physiological model for physiopathology studies or a more rapid NASH model for novel molecule testing.

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The Spanish Journal of Gastroenterology is the official organ of the Sociedad Española de Patología Digestiva, the Sociedad Española de Endoscopia Digestiva and the Asociación Española de Ecografía Digestiva
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